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    您当前的位置:首页 > 技术文章 > ELISA试剂盒实验操作乙醇脱氢酶6抗体的原理

    ELISA试剂盒实验操作乙醇脱氢酶6抗体的原理

    发布时间: 2014-04-15  点击次数: 1303次

    ELISA试剂盒是酶联接免疫吸附剂测定( Enzyme-Linked Immunosorbnent Assay )的简称。它是继免疫荧光和放射免疫技术之后发展起来的一种免疫酶技术。此项技术自70年代初问世以来,发展十分迅速,目前已被广泛用于生物学和医学科学的许多领域。
    英文名称  Anti-ADH6 
    中文名称  乙醇脱氢酶6抗体 
    别    名  ADH 5; ADH 6; Adh6; ADH6_HUMAN; Alcohol dehydrogenase 6 (class V); Alcohol dehydrogenase 6; Aldehyde reductase; Class V alcohol dehydrogenase 6; UDP GLUCURONOSYLTRANSFERASE 2B10 PRECURSOR, MICROSOMAL. 
    浓    度  1mg/1ml 
    规 格  0.2ml/200μg  
    抗体来源  Rabbit  
    克隆类型  polyclonal 
    交叉反应  Human, Mouse, Rat, Pig, Cow  
    产品类型  一抗    
    研究领域  肿瘤 细胞生物 信号转导 新陈代谢  
    蛋白分子量  predicted molecular weight: 39kDa 
    性    状  Lyophilized or Liquid 
    免 疫 原  KLH conjugated synthetic peptide derived from human ADH6 
    亚    型  IgG 
    纯化方法  affinity purified by Protein A 
    储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 
    产品应用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500 
    (石蜡切片需做抗原修复) 
     not yet tested in other applications.
     optimal dilutions/concentrations should be determined by the end user.  
    保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 
    Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
    产品介绍 ADH6 (alcohol dehydrogenase 6), also known as ADH-5, is a 368 amino acid member of the class V zinc-containing alcohol dehydrogenase family. This family of enzymes functions to metabolize a wide variety of substrates such as retinol, hydroxysteroids, ethanol, aliphatic alcohols and lipid peroxidation products. Localized to the cytoplasm and expressed in the stomach and liver, ADH6 catalyzes the reversible oxidation of alcohols to their corresponding aldehydes or ketones and is able to bind two zinc ions as cofactors. ADH6 contains a glucocorticoid response element upstream of its 5' UTR which is thought to be a steroid binding site, suggesting that expression of ADH6 may be under hormonal control. Multiple isoforms of ADH6 exist due to alternative splicing events.
    Subcellular Location : Cytoplasm.
    Tissue Specificity : Stomach and liver.
    Similarity : Belongs to the zinc-containing alcohol dehydrogenase family. Class-V subfamily.
    Database links : UniProtKB/Swiss-Prot: P28332.2
    ELISA试剂盒正式试验时,应分别以阳性对照与阴性对照控制试验条件,待检样品应作一式二份,以保证实验结果的准确性。有时本底较高,说明有非特异性反应,可采用羊血清、兔血清或BSA等封闭。

     

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