ELISA试剂盒试验原理：
试剂盒是固相夹心法酶联免疫吸附实验（ELISA）.已知AOPP浓度的标准品、未知浓度的样品加入微孔酶标板内进行检测。先将AOPP和生物素标记的抗体同时温育。洗涤后，加入亲和素标记过的HRP。再经过温育和洗涤，去除未结合的酶结合物，然后加入底物A、B，和酶结合物同时作用。产生颜色。颜色的深浅和样品中AOPP的浓度呈比例关系。
英文名称  Anti-BRF1/RNA polymerase III 
中文名称  RNA聚合酶III抗体 
别    名  B related factor 1; B-related factor 1; BRF; BRF-1; brf1; brfu; General transcription factor IIIB, 90kD subunit; GTF3B; hBRF; hTFIIIB90; RNA polymerase III; subunit 2; TAF3B2; TAF3C; TATA box-binding protein-associated factor; TF3B_HUMAN; TFIIIB90; Transcription factor IIIB 90 kDa subunit; Transcription initiation factor IIIB. 
浓    度  1mg/1ml 
规 格  0.2ml/200μg  1ml/1mg
抗体来源  Rabbit  
克隆类型  polyclonal 
交叉反应  Human, Mouse, Rat   
产品类型  一抗    
研究领域  细胞生物 染色质和核信号 信号转导 表观遗传学  
蛋白分子量  predicted molecular weight: 74kDa 
性    状  Lyophilized or Liquid 
免 疫 原  KLH conjugated synthetic peptide derived from Human BRF1/RNA polymerase III 
亚    型  IgG 
纯化方法  affinity purified by Protein A 
储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 
产品应用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500 
（石蜡切片需做抗原修复） 
 not yet tested in other applications.
 optimal dilutions/concentrations should be determined by the end user.  
保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 
Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
ELISA试剂盒RNA聚合酶III抗体产品介绍 responsible for the synthesis of a different class of RNA. RNA polymerase I (Pol I) transcribes the rRNA (ribosomal RNA) genes for the precursor of the 28S, 18S, and 5.8S molecules of the ribosome. RNA polymerase II transcribes protein-encoding genes into mRNA (messenger RNA) and snRNA (small nuclear RNA) genes into snRNAs that influence the processing of other classes of RNA. RNA polymerase III (Pol III) transcribes the 5S rRNA genes and all of the tRNA (transfer RNA) genes. Each class of RNA polymerase is assembled from 9 to 15 different polypeptides. The RPB6 and RPB8 subunits are shared by all 3 RNA polymerases.
Function : General activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters. The isoform 1 is involved in the transcription of tRNA, adenovirus VA1, 7SL and 5S RNA. Isoform 2 is required for transcription of the U6 promoter.
Subcellular Location : Nucleus.
Post-translational modifications : Phosphorylated upon DNA damage, probably by ATM or ATR.
Similarity : Belongs to the TFIIB family.
Contains 1 TFIIB-type zinc finger.