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本产品仅用于科研，不用于临床

英文名称 Anti-phospho-RPS6KB1（Ser421）
中文名称 磷酸化核糖体S6蛋白激酶抗体
别 名 RPS6KB1 （phospho S421）; RPS6KB1 （phospho Ser421）; p-RPS6KB1 （Ser421）; RPS6KB1; PS6K; S6K; S6K1; STK14A; p70（S6K）-alpha; p70-S6K; p70-alpha; ribosomal protein S6 kinase; 70kDa; p70 S6 Kinase; P70 Beta1; KS6B1_HUMAN; Ribosomal protein S6 kinase beta-1; S6K-beta-1; 70 kDa ribosomal protein S6 kinase 1; P70S6K1; p70-S6K 1; Ribosomal protein S6 kinase I; Serine/threonine-protein kinase 14A; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6K-alpha; p70 S6KA.
浓 度 1mg/1ml
规 格 0.1ml/100μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit
产品类型 一抗 磷酸化抗体
研究领域 肿瘤 免疫学 信号转导 转录调节因子
蛋白分子量 predicted molecular weight: 57kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human RPS6KB1 around the phosphorylation site of Ser421
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR （mammalian target of rapamycin） signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013].
Function : Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 （mTORC1） leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the preinitiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase （EEF2K）, which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex. The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at 'Thr-412', which is proposed to be a negative feedback mechanism for the RPS6KB1 anti-apoptotic function. Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, resulting in accelerated degradation of IRS1. In cells lacking functional TSC1-2 complex, constitutively phosphorylates and inhibits GSK3B. May be involved in cytoskeletal rearrangement through binding to neurabin.
Subunit : Interacts with PPP1R9A/neurabin-1. Interacts with RPTOR. Interacts with IRS1. Interacts with EIF3B and EIF3C. Interacts with POLDIP3 and TRAF4.
Subcellular Location : Cell junction, synapse, synaptosome.Mitochondrion outer membrane. Mitochondrion. Note=Colocalizes with URI1 at mitochondrion. Isoform Alpha I: Nucleus. Cytoplasm. Isoform Alpha II: Cytoplasm.
Tissue Specificity : Widely expressed.
Post-translational modifications : Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Similarity : Phosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism （By similarity）. Activated by phosphorylation at Thr-252 by PDPK1. Dephosphorylation by PPP1CC at Thr-412 in mitochondrion.
Database links : UniProtKB/Swiss-Prot: P23443.2
宿主：Human、Murine、Rat （Rattus）、Bovine、Dog （Canine）等物种
磷酸化核糖体S6蛋白激酶抗体应用范围: WB, ELISA, IP, IHC （p）, IHC （f）, IF
缓冲液：0.01m PBS,pH值7.4和10毫克/毫升BSA
储存液：0.1%（w/v）*                                                         

TMEM166 跨膜蛋白166抗体Y55555 0.2ml
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phospho-TPH（Ser19） 磷酸化色氨酸羟化酶抗体Y55562 0.1ml
TPO 甲状腺过氧化物酶抗体Y55563 0.2ml
TRA16 睾丸激素受体相关蛋白16抗体Y55564 0.1ml
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TRADD 肿瘤坏死因子受体1相关死亡域蛋白抗体Y55566 0.1ml
phospho-TRADD（Ser299） 磷酸化肿瘤坏死因子受体1相关死亡域蛋白抗体Y55567 0.1ml
phospho-TRADD（Ser269） 磷酸化肿瘤坏死因子受体1相关死亡域蛋白抗体Y55568 0.1ml
phospho-TRADD（Ser274） 磷酸化肿瘤坏死因子受体1相关死亡域蛋白抗体Y55569 0.1ml
TRAF1 肿瘤坏死因子受体相关因子1抗体Y55570 0.2ml
TNFR2/TRAF2/CD120b 肿瘤坏死因子受体相关因子2抗体Y55571 0.1ml
TRAF3/CD40bp 肿瘤坏死因子受体相关蛋白3抗体Y55572 0.1ml
TRAF4/CART 1 肿瘤坏死因子受体相关蛋白4抗体Y55573 0.1ml
TRAF6 肿瘤坏死因子受体相关因子6抗体Y55574 0.1ml
TRAIL 肿瘤坏死因子相关凋亡诱导配体抗体Y55575 0.1ml
Transthyretin 转甲状腺素蛋白抗体Y55576 0.1ml
Transaldolase 1 转醛醇酶抗体Y55577 0.2ml
Triosephosphate isomerase 磷酸丙糖异构酶抗体Y55578 0.2ml
TRBF1/TRF1 端粒体复制结合因子1抗体Y55579 0.1ml
TREM1 髓系细胞触发受体1抗体Y55580 0.2ml
TREM2 髓系细胞触发受体2抗体Y55581 0.1ml
TREML1/TLT1 髓系细胞触发受体样转录因子1抗体Y55582 0.2ml
TREML2/TLT2 髓系细胞触发受体样转录因子2抗体Y55583 0.2ml
Ferritin 铁蛋白抗体Y55584 0.2ml
Transferrin 转铁蛋白抗体Y55585 0.1ml
Transferrin（1F10） 转铁蛋白单克隆抗体Y55586 0.1ml
TRF2 端粒结合蛋白2抗体Y55587 0.1ml
omerase Binding Protein, P23 端粒酶结合蛋白P23抗体Y55588 0.1ml
phospho-p23 （Ser113） 磷酸化端粒酶结合蛋白P23抗体Y55589 0.1ml
TRIM32 TRIM32抗体Y55590 0.1ml
TrK A/NTRK1 酪氨酸激酶A抗体Y55591 0.1ml
Phospho-TrkA （Tyr490）/TrkB （Tyr516） 磷酸化酪氨酸激酶A抗体Y55592 0.1ml
Phospho-TrkA （Tyr674/675）/TrkB （Tyr706/707） 磷酸化酪氨酸激酶A抗体Y55593 0.1ml
Phospho-TrkA （Tyr785）/TrkB （Tyr816） 磷酸化酪氨酸激酶A抗体Y55594 0.1ml
Trk A/B/C 酪氨酸激酶A/B/C抗体Y55595 0.1ml
Trk B/NTRK2 酪氨酸激酶B抗体Y55596 0.1ml
Trk B/NTRK2 酪氨酸激酶B抗体Y55597 0.1ml
phospho-NTRK2/TrkB （Tyr515） 磷酸化酪氨酸激酶B抗体Y55598 0.1ml
phospho-TrkB （Tyr705） 磷酸化酪氨酸激酶B抗体Y55599 0.1ml
Trk C 酪氨酸激酶C抗体（神经生长因子受体的一种）Y55600 0.1ml
Trop-2 原肌球蛋白抗体Y55601 0.1ml
TRP2 酪氨酸酶相关蛋白2抗体Y55602 0.1ml
TRPC6 瞬时受体电位离子通道蛋白6抗体Y55603 0.1ml
Thioredoxin 硫氧还蛋白抗体磷酸化核糖体S6蛋白激酶抗体Y55604 0.1ml