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小鼠β连环蛋白/联蛋白酶免试剂盒,(β-Cat)ELISA检测试剂盒 ,英文名:β-Cat ELISA Kit ,英文缩写:β-Cat ,常见试剂盒规格:96T/48T,产品别名:小鼠β连环蛋白/联蛋白ELISA检测试剂盒,β-Cat 检测试剂盒, β-Cat 检测试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。小鼠β连环蛋白/联蛋白酶免试剂盒,(β-Cat)ELISA检测试剂盒在操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不*,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质。可用洗板机洗板或手工洗板,前者洗涤质量较好,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重复性。
乙二醛酶Ⅰ(GLO1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glyoxalase I (GLO1)48T/96TY13385Homo sapiens (Human)
衰老关键蛋白2(FBLN2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Fibulin 2 (FBLN2)48T/96TY13386Mus musculus (Mouse)
外周蛋白(PRPH)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Peripherin (PRPH)48T/96TY13387Mus musculus (Mouse)
芳烃受体抑制因子(AHRR)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Aryl Hydrocarbon Receptor Repressor (AHRR)48T/96TY13388Homo sapiens (Human)
Slit同源物1(Slit1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Slit Homolog 1 (Slit1)48T/96TY13389Mus musculus (Mouse)
SRC/FGR/YES相关FYN癌基因(FYN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for FYN Oncogene Related To SRC/FGR/YES (FYN)48T/96TY13390Homo sapiens (Human)
磷酸肌醇-3-激酶催化亚基β肽(PIK3Cβ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Phosphoinositide-3-Kinase Catalytic Beta Polypeptide (PIK3Cb)48T/96TY13391Homo sapiens (Human)
血管紧张素Ⅱ(ANGⅡ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Angiotensin II (AngII)48T/96TY13392Oryctolagus cuniculus (Rabbit)
转甲状腺素蛋白(TTR)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Transthyretin (TTR)48T/96TY13393Mus musculus (Mouse)
甲状旁腺激素(PTH)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Parathyroid Hormone (PTH)48T/96TY13394Sus scrofa; Porcine (Pig)
垂体肿瘤转化1相互作用蛋白(PTTG1IP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Pituitary Tumor Transforming 1 Interacting Protein (PTTG1IP)48T/96TY13395Homo sapiens (Human)
白介素2受体α(IL2Rα)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 2 Receptor Alpha (IL2Ra)48T/96TY13396Bos taurus; Bovine (Cattle)
载脂蛋白F(APOF)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Apolipoprotein F (APOF)48T/96TY13397Rattus norvegicus (Rat)
5羟色胺转运蛋白(SERT)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Serotonin Transporter (SERT)48T/96TY13398Mus musculus (Mouse)
全细胞色素C合酶(HCCS)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Holocytochrome C Synthase (HCCS)48T/96TY13399Homo sapiens (Human)
谷氨酸脱羧酶1(GAD1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glutamate Decarboxylase 1, Brain (GAD1)48T/96TY13400Homo sapiens (Human)
Cerberus蛋白(CER)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cerberus (CER)48T/96TY13401Homo sapiens (Human)
谷氨酰胺合成酶(GS)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glutamine synthetase (GS)48T/96TY13402Homo sapiens (Human)
cGMP依赖性蛋白激酶Ⅰ(PRKG1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Protein Kinase, cGMP Dependent Type I (PRKG1)48T/96TY13403Homo sapiens (Human)
胞质分裂作用因子3(DOCK3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Dedicator Of Cytokinesis 3 (DOCK3)48T/96TY13404Homo sapiens (Human)
输出蛋白5(XPO5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Exportin 5 (XPO5)48T/96TY13405Homo sapiens (Human)
胰岛素样生长因子结合蛋白7(IGFBP7)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Insulin Like Growth Factor Binding Protein 7 (IGFBP7)48T/96TY13406Mus musculus (Mouse)
性别决定区Y框蛋白9(SOX9)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Sex Determining Region Y Box Protein 9 (SOX9)48T/96TY13407Homo sapiens (Human)
驱动蛋白家族成员20A(KIF20A)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Kinesin Family, Member 20A (KIF20A)48T/96TY13408Homo sapiens (Human)
含IQ基元GTP酶激活蛋白2(IQGAP2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for IQ Motif Containing GTPase Activating Protein 2 (IQGAP2)48T/96TY13409Mus musculus (Mouse)
酸性葡糖苷酶α(GαA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glucosidase Alpha, Acid (GaA)48T/96TY13410Homo sapiens (Human)
Podocin蛋白(PDCN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Podocin (PDCN)48T/96TY13411Rattus norvegicus (Rat)
激肽原1(KNG1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Kininogen 1 (KNG1)48T/96TY13412Rattus norvegicus (Rat)
肿瘤坏死因子配体超家族成员4(TNFSF4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tumor Necrosis Factor Ligand Superfamily, Member 4 (TNFSF4)48T/96TY13413Homo sapiens (Human)
白细胞免疫球蛋白样受体亚家族B成员4(LILRB4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 4 (LILRB4)48T/96TY13414Homo sapiens (Human)
细胞粘附分子1(CADM1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cell Adhesion Molecule 1 (CADM1)48T/96TY13415Homo sapiens (Human)
核纤层蛋白A/C(LMNA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lamin A/C (LMNA)48T/96TY13416Mus musculus (Mouse)
肝配蛋白B受体3(EPHB3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Ephrin Type B Receptor 3 (EPHB3)48T/96TY13417Mus musculus (Mouse)
醛脱氢酶3家族成员A1(ALDH3A1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Aldehyde Dehydrogenase 3 Family, Member A1 (ALDH3A1)48T/96TY13418Homo sapiens (Human)
转化受体电位阳离子通道亚家族M成员4(TRPM4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Transient Receptor Potential Cation Channel Subfamily M, Member 4 (TRPM4)48T/96TY13419Mus musculus (Mouse)
Klothoβ蛋白(KLβ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Klotho Beta (KLb)48T/96TY13420Mus musculus (Mouse)
多配体蛋白聚糖3(SDC3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Syndecan 3 (SDC3)48T/96TY13421Homo sapiens (Human)
乳腺癌抗雌激素药物耐药性基因1(BCAR1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Breast Cancer Anti Estrogen Resistance 1 (BCAR1)48T/96TY13422Homo sapiens (Human)
肌微管素相关蛋白9(MTMR9)检测试剂盒(酶联免疫吸附试验法)小鼠β连环蛋白/联蛋白酶免试剂盒,(β-Cat)ELISA检测试剂盒 ELISA Kit for Myotubularin Related Protein 9 (MTMR9)48T/96TY13423Mus musculus (Mouse)
皮肤桥蛋白(DPT)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Dermatopontin (DPT)48T/96TY13424Mus musculus (Mouse)
各种类型ELISA测定时,小鼠β连环蛋白/联蛋白酶免试剂盒,(β-Cat)ELISA检测试剂盒 一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。