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小鼠凋亡相关因子酶免试剂盒,(FAS/CD95)ELISA检测试剂盒,英文名:FAS/CD95 ELISA Kit,英文缩写:FAS/CD95 ,常见试剂盒规格:96T/48T,产品别名:小鼠凋亡相关因子ELISA检测试剂盒,FAS/CD95 检测试剂盒,FAS/CD95 检测试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。小鼠凋亡相关因子酶免试剂盒,(FAS/CD95)ELISA检测试剂盒 在操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不*,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质??捎孟窗寤窗寤蚴止は窗?,前者洗涤质量较好,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重复性。
肌球蛋白ⅦA(MYO7A)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Myosin VIIA (MYO7A)48T/96TY14985Homo sapiens (Human)
肌球蛋白ⅨA(MYO9A)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Myosin IXA (MYO9A)48T/96TY14986Homo sapiens (Human)
肌球蛋白Ⅹ(MYO10)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Myosin X (MYO10)48T/96TY14987Homo sapiens (Human)
肌球蛋白ⅩⅤ(MYO15)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Myosin XV (MYO15)48T/96TY14988Homo sapiens (Human)
肌球蛋白ⅩⅥ(MYO16)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Myosin XVI (MYO16)48T/96TY14989Homo sapiens (Human)
原肌球蛋白2β(TPM2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tropomyosin 2 Beta (TPM2)48T/96TY14990Homo sapiens (Human)
原肌球蛋白3(TPM3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tropomyosin 3 (TPM3)48T/96TY14991Homo sapiens (Human)
原肌球蛋白4(TPM4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tropomyosin 4 (TPM4)48T/96TY14992Homo sapiens (Human)
肌球蛋白ⅤB(MYO5B)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Myosin VB (MYO5B)48T/96TY14993Homo sapiens (Human)
肌球蛋白ⅨB(MYO9B)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Myosin IXB (MYO9B)48T/96TY14994Homo sapiens (Human)
肌球蛋白ⅩⅧA(MYO18A)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Myosin XVIIIA (MYO18A)48T/96TY14995Homo sapiens (Human)
肌球蛋白ⅩⅧB(MYO18B)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Myosin XVIIIB (MYO18B)48T/96TY14996Homo sapiens (Human)
丝氨酸蛋白酶23(PRSS3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Protease, Serine 3 (PRSS3)48T/96TY14997Homo sapiens (Human)
神经肽W(NPW)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Neuropeptide W (NPW)48T/96TY14998Homo sapiens (Human)
鞘磷脂合酶1(SGMS1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Sphingomyelin Synthase 1 (SGMS1)48T/96TY14999Homo sapiens (Human)
5-羟色胺受体4(HTR4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for 5-Hydroxytryptamine Receptor 4 (HTR4)48T/96TY15000Homo sapiens (Human)
5-羟色胺受体6(HTR6)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for 5-Hydroxytryptamine Receptor 6 (HTR6)48T/96TY15001Homo sapiens (Human)
乙酰复合胺-O-甲基转移酶(ASMT)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Acetylserotonin-O-Methyltransferase (ASMT)48T/96TY15002Homo sapiens (Human)
5-羟色胺受体7(HTR7)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for 5-Hydroxytryptamine Receptor 7 (HTR7)48T/96TY15003Homo sapiens (Human)
载脂蛋白L2(APOL2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Apolipoprotein L2 (APOL2)48T/96TY15004Homo sapiens (Human)
载脂蛋白L3(APOL3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Apolipoprotein L3 (APOL3)48T/96TY15005Homo sapiens (Human)
前列腺素还原酶1(PTGR1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Prostaglandin Reductase 1 (PTGR1)48T/96TY15006Homo sapiens (Human)
前列腺素还原酶2(PTGR2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Prostaglandin Reductase 2 (PTGR2)48T/96TY15007Homo sapiens (Human)
全羧酶合成酶(HLCS)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Holocarboxylase Synthetase (HLCS)48T/96TY15008Homo sapiens (Human)
甾醇-O-?;泼?(SOAT1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Sterol-O-Acyltransferase 1 (SOAT1)48T/96TY15009Homo sapiens (Human)
二磷酸甘油酸变位酶(BPGM)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Bisphosphoglycerate Mutase (BPGM)48T/96TY15010Homo sapiens (Human)
磷脂酰肌醇蛋白聚糖5(GPC5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glypican 5 (GPC5)48T/96TY15011Homo sapiens (Human)
谷光甘肽合成酶(GSS)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glutathione Synthetase (GSS)48T/96TY15012Homo sapiens (Human)
角蛋白6C(KRT6C)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Keratin 6C (KRT6C)48T/96TY15013Homo sapiens (Human)
犬尿氨酸酶(KYNU)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Kynureninase (KYNU)48T/96TY15014Homo sapiens (Human)
精氨酸加压素原(VP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Preprovasopressin (VP)48T/96TY15015Homo sapiens (Human)
前催产素原(OT)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Preprooxytocin (OT)48T/96TY15016Homo sapiens (Human)
脱氧胸苷酸激酶(DTYMK)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Deoxythymidylate Kinase (DTYMK)48T/96TY15017Homo sapiens (Human)
类胰蛋白酶δ1(TPSδ1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tryptase Delta 1 (TPSd1)48T/96TY15018Homo sapiens (Human)
类胰蛋白酶γ1(TPSγ1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tryptase Gamma 1 (TPSg1)48T/96TY15019Homo sapiens (Human)
甘丙肽受体4(GALR4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Galanin Receptor 4 (GALR4)48T/96TY15020Homo sapiens (Human)
甘丙肽受体3(GALR3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Galanin Receptor 3 (GALR3)48T/96TY15021Homo sapiens (Human)
甘丙肽受体2(GALR2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Galanin Receptor 2 (GALR2)48T/96TY15022Homo sapiens (Human)
金属硫蛋白1X(MT1X)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Metallothionein 1X (MT1X)48T/96TY15023Homo sapiens (Human)
金属硫蛋白1H(MT1H)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Metallothionein 1H (MT1H)48T/96TY15024Homo sapiens (Human)
金属硫蛋白1F(MT1F)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Metallothionein 1F (MT1F)48T/96TY15025Homo sapiens (Human)
金属硫蛋白1G(MT1G)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Metallothionein 1G (MT1G)48T/96TY15026Homo sapiens (Human)
金属硫蛋白1B(MT1B)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Metallothionein 1B (MT1B)48T/96TY15027Homo sapiens (Human)
拓扑异构酶Ⅲ(TOP3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Topoisomerase III (TOP3)48T/96TY15028Homo sapiens (Human)
血小板反应蛋白3(THBS3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Thrombospondin 3 (THBS3)48T/96TY15029Homo sapiens (Human)
滑行蛋白β(TXLNβ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Taxilin Beta (TXLNb)48T/96TY15030Homo sapiens (Human)
磷脂酶D2(PLD2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Phospholipase D2 (PLD2)48T/96TY15031Homo sapiens (Human)
磷脂爬行酶3(PLSCR3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Phospholipid Scramblase 3 (PLSCR3)48T/96T Y15032Homo sapiens (Human)
磷脂爬行酶4(PLSCR4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Phospholipid Scramblase 4 (PLSCR4)48T/96TY15033Homo sapiens (Human)
溶血磷脂酶Ⅱ(LYPLA2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lysophospholipase II (LYPLA2)48T/96TY15034Homo sapiens (Human)
各种类型ELISA测定时, 一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。